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. 2012 Oct 11;7(10):e47091. doi: 10.1371/journal.pone.0047091

Figure 3. Analysis of replication/repair intermediates purified from wild type MMS treated cultures.

Figure 3

A. 2D-gel analysis of replication/repair intermediates from wild type cells (JZ60) during a time course of incubation with 0.03% MMS. Times of MMS incubation are given below the panels. Two different restriction fragments from the rDNA region were analysed. The position of restriction sites and genetic elements of the rDNA region is outlined in the line drawing above the panels. B. Quantification of the ratio (%) of the intensity of repair intermediates and spike intermediates versus the intensity of the Y-arc for the panels shown in A. C. 2D-gel analysis of replication/repair intermediates from wild type cells (JZ60) treated for 1 h with increasing amount of MMS. D. Interpretation of replication/repair intermediates profiles from untreated or MMS-treated wild type cell cultures.