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. 2012 Oct 11;7(10):e47091. doi: 10.1371/journal.pone.0047091

Figure 5. Analysis of replication/recombination intermediates purified from hydroxyurea treated wild type and swi7-1 mutant yeast cells.

Figure 5

A. 2D-gel analysis of replication/recombination intermediates from wild type cells (JZ60) and swi7 (JZ468) during a time course of incubation with 12 mM HU. Times of HU incubation are given to the left of the panels. Two different restriction fragments from the rDNA region were analysed. The position of restriction sites and genetic elements of the rDNA region is outlined in the line drawing above the panels. B. Calculation in ratios (%) of bubble arcs (replication intermediates) and spikes (recombination intermediates) versus Y-arc intermediates.