Figure 8. The rad2 mutation abolishes the presence of the novel alkylation damage repair intermediates.

2D-gel analysis of untreated and MMS treated wild-type and rad2 strains. The HindIII/KpnI fragment of the rDNA was analyzed in the experiment, see Figure 2 line drawing. Cells were treated with 0.03% MMS for 0.5 and 2 h as shown.