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. 2012 Oct 15;23(20):3970–3981. doi: 10.1091/mbc.E12-05-0352

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© 2012 Holt et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — FZR1 regulates protein levels of CDC20, cyclin B1, and BUBR1. (A) Representative immunoblots of GV-arrested oocytes collected from Fzr1^−/− mice or control littermates. At least three separate runs were performed, with n = 50 oocytes per lane. (B) Densitometric analysis of those immunoblots from A. Levels of the APC^FZR1 substrates cyclin B1 and CDC20 were raised, whereas BUBR1 levels were less in Fzr1^−/− oocytes. Error bars show SD.