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. 2012 Oct 15;23(20):3970–3981. doi: 10.1091/mbc.E12-05-0352

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© 2012 Holt et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Premature spindle elongation is independent of CDK1 activity or TPX2, HURP, and Eg5 protein level in Fzr1^−/− oocytes. (A) CDK1 activity measured using an enzyme-linked immunosorbent assay in Fzr1^−/− and control fl/fl oocytes at 2.5 h after GVB. Activity levels were significantly higher in Fzr1^−/− oocytes (*p = 0.037, one-way ANOVA with Tukey's test). Treatment with 2.5 μM flavopiridol reduced CDK1 activity to levels that were not significantly different from control oocytes. (B) Spindle length for control and Fzr1^−/− oocytes, with and without flavopiridol treatment, measured 2.5 h after GVB. Flavopiridol treatment of Fzr1^−/− oocytes did not significantly alter spindle length. (C) Representative immunoblots for TPX2, HURP, and Eg5 on protein from GV-arrested oocytes collected from Fzr1^−/− mice or controls, with n = 50–100 oocytes per lane and at least two separate experiments performed. (D) Densitometric analysis of immunoblots in C. Error bars show SD.