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. 2012 Oct 15;23(20):3982–3992. doi: 10.1091/mbc.E12-07-0535

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© 2012 Saha and Pollard. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Contractile ring assembly in Δmid1 cells depends on formin Cdc12p. Cells were grown and imaged at 25ºC or grown at 36ºC for 6 h (C) and 4 h (D) before imaging at 36ºC. (A–D) DIC and maximum-intensity projections of fluorescence micrographs. Localization of GFP-calponin-homology domain (CHD) of Rng2p and Rlc1p-mCherry in (A) Δmid1 cells, (B) Δfor3 Δmid1 cells, (C) arp2-1 Δmid1 cells at 25 and 36°C, and (D) cdc12-112 Δmid1 cells at 25 and 36°C. Rlc1p-mCherry was expressed from the native locus, and GFP-CHD was expressed from a 41xnmt1 promoter for 24 h. Scale bars, 5 μm.