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. 2012 Oct 15;23(20):4020–4031. doi: 10.1091/mbc.E12-04-0268

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© 2012 Tse et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — NOP-1 regulates polarity establishment. (A) Kymographs of a cortical slice along the AP-axis of control, nop-1(it142), par-2(RNAi), and nop-1(it142);par-2(RNAi) embryos expressing NMY-2::GFP prior to NEBD. The yellow arrow indicates the direction of cortical flow of myosin. (B) Quantification of the size of the metaphase cap of NMY-2::GFP in control and nop-1(it142) embryos. (C) Quantification of the timing of cortical recruitment of GFP::PAR-2 in control, nop-1(RNAi), nop-1(it142), ect-2(RNAi), ect-2(ax751ts), ect-2(ax751ts);ect-2(RNAi), ect-2(ax751ts);nop-1(RNAi), and nop-1(it142);ect-2(RNAi) embryos expressing GFP::PAR-2 and mCherry::PAR-6. Each filled circle represents an individual embryo, and the straight lines represent the average time of recruitment. **, p < 0.01. (D) Kymograph of the segment across the AP-axis of embryos in (C) prior to NEBD.