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. 2012 Oct 15;23(20):4041–4053. doi: 10.1091/mbc.E12-05-0395

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© 2012 Buttery et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — Expression of activated Bnr1 rescues gin4Δ bni1-1 growth defect and actin polarity defect. (A) Expression of bnr1ΔDAD-3HA partially rescues the temperature-sensitive phenotype of a gin4Δ bni1-1 strain. Fivefold dilutions of cells were spotted on yeast extract/peptone/dextrose plates and grown for 1 d at 35°C (bottom) or 2 d at 24°C (top). (B) Myo2-GFP localization is unpolarized in gin4Δ bni1-1 but shows an increased polarization in gin4Δ bni1-1 bnr1ΔDAD-3HA. Images were acquired and analyzed as in Figure 3B. Scale bar, 5 μm. (C) Quantification of Myo2-GFP localization from experiments in B. Cells of the indicated genotype were scored for Myo2-GFP localization to the bud tip (white with dots) or the bud neck (black) or unpolarized localization (gray). Experiments were performed two times (n > 100 cells for each repetition). For gin4Δ bni1-1 bnr1ΔDAD-3HA strains, we analyzed two independent isolates, which both showed similar effects on growth and Myo2-GFP localization.