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. 2012 Oct 11;7(10):e47162. doi: 10.1371/journal.pone.0047162

Figure 5. Bridging of nucleosomes by PSC does not require the acidic patch on histone H2A.

Figure 5

(a) Schematic diagram of nucleosome bridging assay. (b) Representative control reactions for nucleosome bridging (mock MNase digested) showing that PSC binds both rec-WT and H2A-STT chromatin. Arrows point to the main plasmid forms (nicked and supercoiled); the array of minor isoforms observed here is atypical but the isoforms behave similarly. (c) Representative MNase digested nucleosome bridging reactions demonstrating that PSC bridges both rec-WT and H2A-STT nucleosomes. (d) Summary of nucleosome bridging assays on chromatin templates assembled with recombinant wild-type (rec-WT) and H2A acidic patch mutant histones (H2A-STT). Values from fractions 5–7 (bottom fractions) of sucrose gradients were summed and plotted.