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. 2012 Oct 11;6(10):e1856. doi: 10.1371/journal.pntd.0001856

Figure 2. Mucins are less prone to degradation during acute infection.

Figure 2

Mucus isolated from mice on day 21 of acute (A, B) and chronic (C, D) infection was treated with 50 µg/ml of ESPs for 24 h and subjected to 6–8 M GuCl rate zonal centrifugation. (E, F) Mucus isolated on day 42 of acute (Ac) and chronic (Chr) infection was analysed by rate zonal centrifugation. Subsequently tubes were emptied by taking fractions from the top of the gradient (low density, smaller molecules) and analysed by PAS staining; data presented as staining intensity (a.u). Data are also presented as a percentage of AUC of Fr1–8, 9–14 and 15–18 from untreated (−ES) and ESP-treated (+ES) mucus. Results represent the mean of 3–5 mice ± SD ** = P<0.01.