Figure 2. L. (V.) braziliensis isolates differ in their ability to hydrolyze adenine nucleotides.

Promastigotes were isolated on the 5th day of culture and incubated with ATP (A), ADP (B) and AMP (C) for 1 hr at 30°C. Enzymatic activity was evaluated by the measurement of inorganic phosphate released. Bars represent the mean+standard deviation (SD) of three or more independent experiments performed in triplicates. Significant differences are shown below each graph. Statistical analysis was performed by one-way ANOVA followed by Bonferroni post-test.