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. 2012 Oct 11;6(10):e1850. doi: 10.1371/journal.pntd.0001850

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© 2012 Leite et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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BMDC obtained after 9 days of culture with GM-CSF were infected with CFSE-labeled metacyclic promastigotes (3 parasites/cell). After 3 hr, the cells were stimulated with 2 µg/mL LPS and then incubated for up to 17 hr. Finally, DC were analyzed by flow cytometry. DC were gated into populations of uninfected (CFSE⁻ cells) and infected (CFSE⁺ cells) BMDC, and the surface markers MHCII and CD86 analyzed in both populations. (A) Percentage of CFSE⁺ DC. (B) Percentage of MHCII⁺CD86⁺ DC. (C) Reduction of MHCII⁺CD86⁺ cells in the population of infected DC compared to uninfected DC. Bars represent the mean+SD of three independent experiments. (*) indicates statistical difference (p<0.05). Statistical analysis was performed by Students's t-test.