Figure 3. The purity of CD34⁺ separation decreases with an increasing number of MNC in CCB.

MNC were isolated from CCB or FCB by washing with DNAse buffer or Ficoll-paque density gradient centrifugation as described in materials and methods. CD34⁺ stem cells were isolated from MNC by a positive magnetic separation of CD34⁺ cells and the purity was determined by flow cytometry. The correlations between the number of MNC and the purity of the CD34⁺ separation for CCB (A; no significance) and FCB (B) are shown. Correlations were evaluated using GraphPad Prism software and the Spearman rank correlation coefficient method.