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(A), (B) DVS reaction time-course of TT1 and TT2, respectively. 8 µg of each aliquot-sample was electrophoresed after blocking the reaction with 0.2 M β-mercaptoethanol, final concentration. (C), (D) DFDNB reaction of BS-RNase tetramers and RNase A dimers analyzed under reducing and non-reducing conditions, respectively. Samples were concentrated to 1 mg/ml, and 10 µg of each were analyzed.
