Figure 4. SEC chromatograms of K113N BS-RNase aggregates obtained by lyophilising the protein from a 40% (v/v) acetic acid solution.

(A), Superdex 75 chromatogram of the mutant (blue) overlapped with a chromatogram of wt BS-RNase (red). (B), SEC of K113N BS-TT₁ and TT₂ gathered together immediately after their elution from the aggregates mixture (day 0-blue curve) and re-cromatographed after storage in 0.2 M NaPi, pH 6.7, for one, two, or three days (1-red, 2-dark green, 3-pink curves, respectively).