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. 2012 Oct 11;7(10):e46804. doi: 10.1371/journal.pone.0046804

Figure 7. Action of BS-RNase oligomers on the proliferation of mesenchimal VIT1 cells.

Figure 7

(A) wt, and (B) K113N BS-RNase. Cells were cultured in RPMI 1640 medium supplemented with 2 mM glutamine, 10% FBS, and 50 µg/ml gentamicin sulphate. After addition of the BS-RNase species, 10 to 240 µg/ml of BS-RNase (native) dimer, or of 10 to 80 µg/ml of BS TT1/C, TT2/N, or a mixture of larger oligomers (L.O.), cells were incubated for 72 h at 37°C with 5% (v/v) CO2. At the end of the treatments cells were stained with a Crystal Violet solution and the survival was measured, and compared to the control lacking any RNase species, as reported in Materials and Methods. Experiments were performed in triplicate; the S.D. are comprised between 4.5 and 6.0% for wt (A), and between 5.1 and 7.7% for K113N BS-RNase (B).