Fig. 3.

A: PGP 9.5-immunostaining of the distal colorectal wall. PGP 9.5-immunoreactive nerve fibers were quantified as area (proportion) with respect to colorectal mucosal and submucosal areas (B), which were normalized by control mean PGP 9.5 proportions and displayed in C. Intracolonic TNBS significantly reduced the density of PGP 9.5-immunoreactive nerve fibers on day 2, which was partially restored by day 14 (compare parts 1 and 2 on day 14) and returned to normal on day 28. *P < 0.05; **P < 0.01; †P < 0.001.