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. 2012 Jul 31;303(8):E1025–E1035. doi: 10.1152/ajpendo.00107.2012

Fig. 3.

Fig. 3.

Influence of Adp27 on Fen-induced cell proliferation and CDK2 mRNA expression in UtSMCs at 24 h. A: p27 expression measured by Western blot analysis. Bars from left to right: control, Fen, Adp27, Adp27 + Fen, Adp27 + Dox, and Adp27 + Dox + Fen. Note that p27 expression was decreased by 10 μM Fen in noninfected cells. p27 was overexpressed in Adp27-infected cells. In the presence of Dox, exogenous expression of p27 was turned off. B: cell counts. Cell numbers were increased after 10 μM Fen in noninfected cells. In Adp27-infected cells, this effect was abolished. In the presence of Dox, Fen-induced increased cell numbers were restored. C: BrdU incorporation. incorporation of BrdU was increased after 10 μM Fen in noninfected cells. In Adp27-infected cells, this effect was abolished. In the presence of Dox, Fen-induced increased BrdU incorporation was restored. D: CDK2 mRNA expression. CDK2 mRNA expression was increased after 10 μM Fen in noninfected cells. In Adp27-infected cells, this effect was abolished. In the presence of Dox, Fen-induced increased CDK2 mRNA levels were restored. All experiments were repeated at least 3 times with independent cell cultures. Fold changes were standardized to controls. *P < 0.05 vs. control group; **P < 0.05 vs. Adp27 + Dox group. Error bars represent SE; n ≥ 3.