Fig. 1.

L6-GLUT4myc cells were stably transfected with either an empty lentiviral vector or a tribbles 3 (TRIB3) short-hairpin RNA (shRNA) lentiviral vector. A: knockdown (KD) of TRIB3 protein in L6 muscle cells. B: basal (open bars) and insulin-stimulated (filled bars) glucose uptake rates were measured; values are means ± SD. C–E: L6 cells were stably transfected with either an empty lentiviral vector or a TRIB3 shRNA lentiviral vector and analyzed by Western blot for phosphorylated insulin receptor substrate-1 (p-IRS-1) at the Tyr⁶¹² site (C), p-Akt at the Ser⁴⁷³ site (D), and p-ERK (E) with or without 45 min of 100 nM insulin stimulation. *P < 0.05. Values are means ± SD for 3 repeated experiments.