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Fasting and short-term nutrient excess have opposite effects on TRIB3 expression in rat skeletal muscle. RNA and protein were isolated from rat skeletal muscle samples from control (normal chow), fasted, and Western diet-fed groups. Real-time RT-PCR analysis of TRIB3 mRNA level (A) and Western blot analysis of TRIB3 protein levels (B) were measured in all groups. *P < 0.05. Results are expressed as means ± SD; n = 6.
