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. 2012 Aug 17;303(8):L720–L727. doi: 10.1152/ajplung.00409.2011

Fig. 2.

Fig. 2.

Ubiquitination assay in CFBE-wild-type (wt) cells transfected with siNeg or siNedd4–2. A–C: immunoprecipitation (IP) was conducted with either an anti-CFTR antibody (M3A7) or a control antibody (IgG), and Western blots (WB) were probed for Nedd4–2 (A), ubiquitin (FK2 antibody, B) or wt-CFTR (596 antibody, C). siNeg and siNedd4–2 samples were run on the same gel but are separated for presentation. D: summary of Nedd4–2 levels in siNeg and siNedd4–2 experiments (N = 3). E: summary of the effects of siNedd4–2 on ubiquitinated CFTR, which is reported as the amount of ubiquitinated CFTR (FK2) divided by the amount of total CFTR determined by Western blot (N = 3). ***P < 0.001.