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Coimmunoprecipitation experiments with CFBE-wt cells. IP was conducted with either an anti-CFTR antibody (C-term) or a control antibody (IgG). A: WB were probed for wt-CFTR (596 antibody, top), Nedd4–2 (middle), or USP10 (bottom). B: Western blots of cells overexpressing Nedd4–2 were probed for CFTR (596 antibody, top) and Nedd4–2 (bottom).
