Fig. 5.

For OVA-challenged mice, 5-HTP supplementation did not alter expression of lung cytokines, chemokines, growth factors, or mucin. The lungs and BAL were collected from the OVA-challenged mice in Fig. 3A. A: BAL supernatants from lungs in Fig. 3A were tested for levels of cytokines using the Luminex 20plex cytokine kit. Not detected were IFN-γ, IL-1α, IL-1β, IL-2, KC, monocyte chemoattractant protein (MCP)-1, and TNF-α; IL-17 was very low (data not shown). B: lung tissue from mice in Fig. 3A was placed in RNAlater and then examined by quantitative PCR for IL-4, IL-5, IL-13, IL-17, CCL11, CCL24, and MUC5AC expression. n = 8–10 mice/group. *P < 0.05 compared with the saline groups. There is not a significant effect of 5-HTP supplementation of OVA-treated mice compared with control diet OVA-treated mice.