Fig. 1.
Detection of lynx1 and α7 nicotinic acetylcholine receptor (nAChR) in monkey lung and bronchial epithelial cells. A: immunohistochemical detection of α7 nAChR (inset, nonimmune serum control), lynx1, and colocalization in monkey lung. Calibration bar represents 35 μm. Inset: box in merge shows high-power view. B: immunohistochemical detection of lynx1, α7 nAChR, and colocalization in primary cultures of rhesus bronchial epithelial cells (BEC). Calibration bar represents 60 μm. C: lynx1 and α7 nAChR form a complex in BEC. a: Western blot of total α7 nAChR and lynx1 in BEC. b: Western blot of α7 nAChR and lynx1 after immunoprecipitation with anti-α7 monoclonal antibody (mouse) or nonimmune serum showing that α7 and lynx1 form a complex in BEC. Similar results were seen if the antibody for lynx1 was used for the immunoprecipitation and the α7 antibody was used for the Western blot (data not shown). In addition, no lynx1 was seen after immunoprecipitation with an unrelated antibody (anti-GABAAR 2/3) (data not shown). The experiment was repeated 4 times.