Fig. 3.
Lynx1 modulates the downstream effects of α7 nAChR signaling in BEC. A and B: siRNA knockdown of lynx1 increased the nicotine-induced upregulation GABAAR α5 and GABAAR β2 mRNA expression (n = 8 per group). C and D: Western blot analysis showed that changes in protein levels mirrored changes in RNA levels (n = 4 per group). E and F: siRNA knockdown of α7 nAChR blocked the effect of the lynx1 knockdown on the nicotine-induced increases in GABAAR α5 and GABAAR β2 mRNA levels (n = 5 per group). The values are expressed as relative fold change of each condition vs. control. Error bars show SE (*P < 0.05, †P < 0.01 by t-test).