Fig. 4.

Lynx1 modulates the ability of nicotine to activate Src signaling. A: representative Western blot showing knockdown of lynx1 increased basal and nicotine-stimulated (2 μM nicotine × 48 h) levels of Src and p-Src. Knockdown of α7 prevented the increase in Src and p-Src caused by the lynx1 knockdown. As shown in B and C, increases in p-Src primarily reflect increases in total Src, as levels of both c-Src and p-Src are increased. Images are representative from 4–5 similar experiments that were pooled for the densitometric analyses. *P < 0.05 compared with corresponding control by Fisher's multiple-comparison tests after 1-way ANOVA.