Fig. 5.
Src mediates regulation of GABAAR and mucin mRNA expression by nicotine and lynx1. A: Src inhibitor PP2 (1 μM) decreased the ability of nicotine (1 μM) and lynx1 knockdown to increase GABAAR α5 mRNA expression in cultured BEC. By comparison, the protein kinase C inhibitor GF 109203X (GF) had no effect. *P < 0.05 for nicotine + siRNA-treated group compared with groups shown by Fisher's multiple-comparison tests after 1-way ANOVA. B: nicotine-induced increase in mucin mRNA was blocked by PP2. PP2 also blocked the additional increase in mucin mRNA caused by lynx1 knockdown. *P < 0.05 for nicotine + siRNA-treated group compared with groups shown by Fisher's multiple-comparison tests after 1-way ANOVA (n = 5 per group). Drugs and siRNAs were added to cultures 48 h before harvesting of cells.