Fig. 2.

Lysyl oxidase/ lysyl oxidase-like protein (LOX/LOXL) family member expression 7 days after ANG II infusion in the presence of a high-fat diet. Quantitative RT-PCR was performed on cDNA from aortic tissue of ApoE^−/− mice. A–C: LOX, LOXL1, and LOXL2 expression were not affected by ANG II in the presence of a high-fat diet. D: LOXL3 expression increased with ANG II on an atherogenic diet. *P = 0.005. E: Western blot analysis on ApoE^−/− mice 7 days after treatment with low-fat diet or high-fat diet + ANG II. By Western analysis, LOX and LOXL3 were the only detectable family members in the low-fat and high-fat diet + ANG II setting 7 days post-treatment. Neither LOX nor LOXL3 showed a statistically significant change in expression with treatment (n = 3).