Table 1.
cDNA Targets | Primer Sequences | Annealing, °C |
---|---|---|
LOX | 5′ TAGCGAAGCACATAGCATTG 3′ | 60 |
5′ TGCAGCAATGAGTCTACAGC 3′ | ||
LOXL1 | 5′ CTATGCCTGCACCTCTCACA 3′ | 61 |
5′ TGGACGATTTTGCAGTTTGT 3′ | ||
LOXL2 | 5′ GAGTGGAGGTGCTAACAGAG 3′ | 59 |
5′ AGTCTGCTGGACAATCTCAG 3′ | ||
LOXL3 | 5′ ACTGTACCCATGATGAGGAT 3′ | 57 |
5′ GCCATGTTGACTCTCTTTTC 3′ | ||
LOXL4 | 5′ CTGGCGTTGCTTGTATGAACA 3′ | 64 |
5′ AGACAGAAGCTGGCCTTGTGT 3′ | ||
PAI-1 | 5′ ACAGCCAACAAGAGCCAATC 3′ | 64 |
5′ GACACGCCATAGGGAGAGAA 3′ | ||
18S | 5′ Gaacgtctgccctatcaact 3′ | 64 |
5′ ccaagatccaactacgagct 3′ |
LOX/LOXL, lysyl oxidase/lysyl oxidase-like proteins.