Fig. 6.

IR-induced cardiomyocyte apoptosis was attenuated by TRV120023 pretreatment. A: representative images of terminal deoxynucleotidyl transferase-mediated nick end-labeling (TUNEL) staining. White arrowheads indicate TUNEL-positive nuclei (green). B: the number of TUNEL-positive nuclei was increased significantly in IR groups compared with Sham groups. TUNEL-positive nuclei were significantly attenuated in TRV120023-treated groups (*P < 0.001 for all IR vs. Sham conditions and for TRV IR vs. losartan and saline IR; n = 5–6/group). C: the antiapoptotic effect of TRV120023 was lost in β-arrestin-2 KO animals undergoing IR (**P < 0.05 for TRV Sham vs. IR condition; n = 4–6/group). TRV = 100 μg·kg⁻¹·min⁻¹ infusion; losartan = 5 mg·kg⁻¹·min⁻¹ infusion.