Fig. 7.

Effect of cytochalasin D (CD, 2 μM) treatment for 8 h on Thr⁶⁴¹ phosphorylation of PKCβ₂ (A), PKCβ₂ membrane translocation (B), ROCK activity (C), iNOS expression (D), RhoA expression (E), and Ser¹⁸⁸ phosphorylation (F) in cardiomyocytes isolated from diabetic rat hearts. G: representative Western blot of the effect of C3 exoenzyme or cytochalasin D treatment for 24 h on the F-to-G-actin ratio in isolated adult rat cardiomyocytes and photomicrographs of isolated adult cardiomyocytes treated for 24 h with C3 exoenzyme or cytochalasin D. *P < 0.05 compared with the other groups (n = 3–4).