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. 2012 Aug 15;303(8):C862–C871. doi: 10.1152/ajpcell.00338.2011

Fig. 6.

Fig. 6.

VIP17/MAL overexpression results in aberrant ciliogenesis in an in vitro three-dimensional cyst culture model. MDCK cells suspended in Matrigel were analyzed by immunofluorescence to localize acetylated tubulin (green). Nuclei are labeled with propidium iodide (red). Prominent cilia are seen protruding into the apical lumen in WT MDCK cysts (top), while very few, shortened cilia are seen in VIP17/MAL overexpressing cysts (bottom). Arrows point to multilumen cysts.