Table 2.
Primer sequences used to clone the rat Sertoli cell CAR full-length cDNA and its insertion into pCI-neo mammalian expression vector*
| Gene | Primer Sequence | Orientation | Position | Length, bp | Tm, °C | Cycle No. | GenBank Accession No. |
|---|---|---|---|---|---|---|---|
| Ex-CAR | 5′-CTGAGAGCGTTTACCTGC-3′ | Sense | (−40)–(−23) | 1,288 | 54.3 | 35 | NM_053570 |
| 5′-TGAGGCAGACAACAGGAT-3′ | Anti-sense | 1,231–1,248 | |||||
| CAR | 5′-ACCTCGAGATGGCGCTCCTACTGTG-3′ | Sense | 1–17 | 1,059 + 8 + 10 = 1,077 | 56 | 35 | NM_053570 |
| 5′- ACGCGGCCGCTTATACCACTGCAATGCCATCG-3′ | Anti-sense | 1,038–1,059 |
*
CTCGAG, restriction site for XhoI; GCGGCCGC, restriction site for NotI; ATG, start codon; TTA, stop codon; Tm, annealing temperature (see Fig. 1 for the primary sequence of rat testicular CAR).