Fig. 4.

Whole cell voltage-clamp recordings from an HL-1 mouse cardiomyocyte. A: time-dependent inward and outward currents recorded in response to voltage steps from −60 to 80 mV (10-mV increments) and a holding potential of −80 mV. B: repetition of the voltage protocol in the same cell as in A but 10 min after added LPS endotoxin (S. enteritidis; 1 μg/ml). C: amplitude of steady-state outward currents (at arrows in A and B) vs. the clamped voltage before voltage-step back to holding potential = −80 mV. ●, Control; ○, 10 min following added LPS endotoxin (S. enteritidis; 1 μg/ml); means ± SE (n = 3); *P < 0.05; #P < 0.01. Time and current calibration applies to A and B. LBP (10 ng/ml) was added in all measurements.