Fig. 3.

Effects of CFTR inhibition on apical HCO₃⁻ efflux in wild-type and Slc26a6^−/− ducts luminally perfused with high HCO₃⁻ solution. The lumen was perfused with the high-HCO₃⁻ solution containing 125 mM HCO₃⁻ and 24 mM Cl⁻. Cells were stimulated with forskolin and alkali-loaded with an acetate prepulse. A: changes in pH_i in the presence (thick line) and absence (thin line) of luminal CFTRinh-172 in wild-type ducts. Representative traces from 8 experiments. B: changes in pH_i in the presence (thick line) and absence (thin line) of luminal CFTRinh-172 in Slc26a6^−/− ducts. Representative traces from 7 and 9 experiments, respectively. C: The recovery rate of pH_i in the presence (solid bar) and absence (open bar) of luminal CFTRinh-172 in wild-type and Slc26a6^−/− ducts.