Fig. 5.
Cl−-independent apical HCO3− efflux in Slc26a6−/− and ΔF ducts. Ducts were superfused with the Cl−-free HCO3−-buffered solution containing forskolin. Lumen was microperfused with the Cl−-free, HCO3−-free solution. Cells were alkali loaded with an acetate prepulse. A: changes in pHi in wild-type (thin line) and Slc26a6−/− ducts (thick line). Representative traces from 7 and 8 experiments respectively. B: data are means ± SE of the recovery rate of pHi. *P < 0.05, significant difference compared with wild-type ducts. C: changes in pHi in wild-type (thin line) and ΔF/ΔF ducts (thick line). Representative traces from 7 experiments. D: data are means ± SE of the recovery rate of pHi. *P < 0.05, compared with wild-type ducts.