Fig. 6.

Effects of luminal H₂DIDS and Cl⁻ removal on membrane potential in wild-type and Slc26a6^−/− ducts luminally-perfused with high Cl⁻ solution. Bath and lumen were perfused with the standard HCO₃⁻-buffered solution containing 25 mM HCO₃⁻ and 124 mM Cl⁻. Cells were stimulated with forskolin. Membrane potential (Pd) was measured by conventional microelectrodes. A and B: H₂DIDS (200 μM) was applied to the lumen as indicated in wild-type (A) and Slc26a6^−/− (B) ducts. Representative of 4 experiments. C and D: Cl⁻ in the luminal solution was replaced with glucuronate as indicated in wild-type (C) and Slc26a6^−/− (D) ducts. Representative of 4 experiments.