Figure 5.

MicC in 5′P Form Can Be Detected In Vivo

(A) Northern blot analysis of sRNAs from total RNA extracts of wild-type strain of Salmonella. The top panel shows the results for MicC at different cell growth densities (LOG, exponential phase, ES, early stationary phase; S, three hours into stationary phase). The same amounts of total RNA were either treated (+) with Terminator Exonuclease (TEX), which degrades RNA with a 5′ monophosphate, or untreated (−). Controls are shown for the sRNAs InvR, which is less TEX sensitive in logarithmic and exponential phases (middle panel), and ArcZ, which carries a 5′ monophosphate group and is sensitive to TEX treatment (bottom panel).

(B) Bar chart representing the monophosphorylated fraction of sRNAs analyzed (MicC-blue, InvR-red, ArcZ-green), calculated from the percentage of integrated signal relative to the control not treated with TEX. Error bars were estimated from three independent experiments. (See also Figure S10.)