Figure 2.

Crystal Structure Docking and Neck-AAA4 Interaction

(A) Ribbon representation of the D. discoideum cytoplasmic dynein crystal structure in the presence of ADP (PDB 3VKG; Kon et al., 2012) docked into the C. reinhardtii axonemal dynein-c cryo-EM map prepared in the absence of nucleotide (transparent surface). Arrow depicts the difference in linker position between these structures obtained in different nucleotide states.

(B) Docking of the S. cerevisiae cytoplasmic dynein crystal structure prepared in the absence of nucleotide (PDB 4AKI; Schmidt et al., 2012). Schematics show the nucleotide occupancy in the crystal structures (D, ADP; T, ATP).

(C) Rotated view, showing the interaction between the linker and AAA5 and a novel contact between the neck subdomain of the tail and AAA4.