Figure 7.

Dynein-c Structure in the Axoneme

(A and B) Views of the primed dynein-c model (Figure 6B) docked into an averaged tomogram of ADP.Vi-dynein-c in the axoneme (Movassagh et al., 2010) (wire mesh). The quality of the fit is best seen in Figure S5A. The linker domain is colored in magenta. Plus (+) and minus (−) signs show microtubule polarity; plus ends are at the tip of the axoneme. The “A” and “B” microtubules in each doublet are labeled.

(C) View of the unprimed dynein-c model docked into an averaged tomogram of dynein-c in the axoneme without nucleotide (wire mesh) (Bui et al., 2009).

(D) Overlay, superposing the tips of the stalks of the two dynein-c models, with nondynein-c densities removed for clarity. The arrow depicts the inferred displacement of the “cargo” microtubule doublet past the adjacent “track” doublet.

The view in (B)–(D) is from the outside of the axoneme, looking in. The view in (A) is from the base of the axoneme, looking toward the tip.