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. 2001 Jun 19;98(13):7510–7515. doi: 10.1073/pnas.131195198

Figure 1.

Figure 1

DHA suppresses both TPA- and EGF-induced AP-1 activity, but EPA suppresses only TPA-induced AP-1 activity. AP-1 luciferase reporter plasmid stably transfected JB6 P+ 1-1 cells were cultured and treated as described in Materials and Methods. After a 48-h treatment with fatty acids at the concentrations indicated, 20 ng/ml TPA or EGF was added and the cells were cultured for another 24 h before harvest. (A) DHA at 2.5 μg/ml significantly inhibits TPA-induced AP-1 activity (67% inhibition, P < 0.01; mean ± SD of triplicate experiments, six wells each); increased concentrations of DHA did not further inhibit AP-1 activity. EPA inhibited TPA-induced AP-1 activity in a concentration-dependent manner (P < 0.01; mean ± SD of triplicate experiments, six wells each). AA failed to inhibit AP-1 activity at the concentrations indicated (P > 0.05; mean ± SD of triplicate experiments, six wells each). (B) DHA inhibited EGF-induced AP-1 activity in a concentration-dependent manner (P < 0.01 at 10 or 20 μg/ml; mean ± SD of triplicate experiments, six wells each). Neither EPA nor AA affected EGF-induced AP-1 activity (P > 0.05; mean ± SD of triplicate experiments, six wells each).