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AA abrogates the DHA inhibitory effect on TPA- or EGF-induced cell transformation. JB6 Cl 41 cells from triplicate wells were exposed to 20 ng/ml TPA or EGF with 20 μg/ml DHA and increasing concentrations of AA as indicated. Cells were incubated for another 4 weeks as described in the legend of Fig. 3. AA effectively abrogated the inhibitory effects of DHA on both TPA- and EGF-induced cell transformation on soft agar (P < 0.01; n = 3).
