Figure 2.

Phylogeny of PILS proteins. The phylogenetic tree of PILS proteins can be divided into three clades: Clade I (blue), Clade II (green), and Clade III (red). The Arabidopsis PILSes are found in the Clade II and Clade III, while Clade I is represented only by PILS proteins from algae. The Maximum Likelihood molecular phylogenetic analysis was performed in MEGA5 (Tamura et al., 2011) by using 42 amino acid PILS sequences from algae, Physcomitrella, Selaginella, Brachypodium, Oryza, and Arabidopsis as explained in the Materials and Methods.