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PCR amplification of DNA with primers ITS1, ITS4 (first primer pair) and with primers ITS3, ITS4, or primers ITS86, ITS4 (second primer pair). Lanes: 1, A. flavus; 2, A. fumigatus; 3, C. albicans; 4, Fusarium species; and 5–8, clinical specimens from four patients. N, negative control; M, size marker of 100-bp ladder standard DNA.
