Mating affects egg laying by G. atropalpus but not autogenous egg maturation. (A) Oocyte yolk deposition (mean and s.e.m. yolk length/oocyte) in virgin and mated females at 24, 48 and 72 h PE (F5,97=188.9, P<0.0001). Statistical comparisons performed as indicated in Fig. 1A. (B) Mature follicles (observed at 72 h PE) and eggs oviposited by virgin and mated females (mean and s.e.m. number/female). No difference was observed in follicle numbers, but mated females laid significantly more eggs (*) than virgin females (t-tests, α≤0.01, N=16–20 females per treatment).