Table 1:

The sequence of primer sets for human papilloma virus (HPV) and beta globin gene used in a touch down PCR program

General HPV primer set		Product size
Forward	5-TTT GTT ACT GTG GTA GAT AC	140 bp
Reverse	5-GAA AAA TAA ACT GTA AAT CA
Beta globin gene specific primer set		Product size
Forward	5-ACA CAA CTG TGT TCA CTA GC	100 bp
Reverse	5-CAA CTT CAT CCA CGT TCA CC
PCR Program
-Initial denaturation		94 C for 3 min
-16 cycles with	-denaturation at	94C for 30 seconds
	-anealing at	65C to 50C for 2 minutes
	-extension at	72C for 30 seconds
-14 cycles with	-denaturation at	94C for 30 seconds
	-anealing at	50C for 1 minutes
	-extension at	72C for 30 seconds
-10 cycles with	-denaturation at	94C for 30 seconds
	-anealing at	49C to 40C for 1 minutes
	-extension at	72C for 30 seconds
-Final extension		72C for 5 minutes