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Chronic blockade of CB1 receptors increased embryonic limb movements in ovo. Plot of the average embryonic kicking time in ovo in 5 min observation periods during the 2 d treatment with vehicle (DMSO black) and 5 μm AM 251 dissolved in DMSO (gray). Limb movements started to increase in AM 251-treated embryos 12 h after injection and remained more active than controls during the rest of the 2 d long treatment.
