Table 1.

Effect of cannabinoid modulators on motoneuron resting membrane properties and on the mPSC parameters

Parameter	Control (n = 7)	AM 251 (n = 7)	Control (n = 15)	WIN 55,212-2 (n = 17)	Control (n = 9)	ACEA (n = 6)	Control (n = 5)	AM 404 (n = 5)	Control (n = 8)	LY 2183240 (n = 13)
Membrane potential (mV)	−63 ± 9.2	−66 ± 8.7	−65 ± 5.4	−71 ± 4.7	−71 ± 5.3	−65 ± 4.2	−59 ± 6.4	−62 ± 7.1	−61 ± 3.5	−60 ± 4.2
Membrane resistance (MΩ)	519 ± 76	487 ± 53	582 ± 56	573 ± 28	576 ± 89	521 ± 49	448 ± 62	472 ± 85	519 ± 38	530 ± 27
AMPA mPSC mean amplitude (pA)	8.4 ± 0.2	8.5 ± 0.5	15.2 ± 1.5	15.6 ± 1.4	16.8 ± 0.4	11.0 ± 0.3	8.8 ± 0.5	9.1 ± 1.5	12.8 ± 0.6	12.9 ± 0.7
AMPA mPSC frequency (Hz)‡	0.24 ± 0.03	1.03 ± 0.03	0.26 ± 0.08	0.10 ± 0.02	0.88 ± 0.24	0.4 ± 0.08	0.4 ± 0.04	0.11 ± 0.02	0.45 ± 0.14	0.15 ± 0.05
	(n = 7)	(n = 7)‡	(n = 8)	(n = 8)‡	(n = 4)	(n = 4)‡	(n = 5)	(n = 5)‡	(n = 5)	(n = 5)‡
AMPA mPSC decay time (ms)	4.8 ± 0.2	5.2 ± 0.3	4.9 ± 0.7	5.1 ± 0.6	6.7 ± 0.3	7.1 ± 0.4	7.4 ± 1.0	9.3 ± 1.1	5.6 ± 0.3	6.6 ± 0.5
GABA mPSC mean amplitude (pA)	13.3 ± 0.4	12.0 ± 0.6	23.7 ± 2.1	25.3 ± 2	24.4 ± 1.1	14.5 ± 0.5	17.8 ± 1.8	11.5 ± 1.4	n.m.	n.m.
GABA mPSC frequency (Hz)	0.57 ± 0.04	0.61 ± 0.04	0.28 ± 0.1	0.31 ± 0.08	0.57 ± 0.06	0.54 ± 0.07	0.52 ± 0.07	0.35 ± 0.11	n.m.	n.m.
GABA mPSC decay time (ms)	26.3 ± 0.8	27.4 ± 1.1	32 ± 4	33.6 ± 2.7	32.8 ± 0.1	35.4 ± 0.1	30.8 ± 1.3	34.7 ± 1.1	n.m.	n.m.

Bold numbers indicate significant differences (p < 0.05), n.m. indicates not measured.

^‡Denotes that the value was obtained from the same cells before and during drug addition (number of cells shown in parentheses). Averages are expressed as mean ± SEM Student's t test (α = 0.05) was used to perform the statistical analyses of membrane potential, resistance, mPSC amplitude, frequency, and decay time.