Figure 3.

L + E + P is the most potent combination for inhibition of cell migration and chemotaxis toward SDF1α as well as increasing cell adhesion of hormone-independent prostate cancer cells. PC3 cells were treated with different combination of PJ components L, E, and P at (A) 4 and (B) 8 µg/ml for up to 72 hours, and migration assay was performed as described in Figure 1. (C) PC3 cells were plated on gelatincoated dishes, and 24 hours later, the medium was changed and the cells were treated with PJ components L + E + P at 4 and 8 µg/ml. Adhesion assay was performed as described in Figure 1. (D) PC3 cells were allowed to attach to the top of the filter of the chemotaxis chamber for 4 hours and then treated with L + E + P at 4 and 8 µg/ml for 12 hours. Chemotaxis assay was performed as described in Figure 1. Bars represent SEM. ***P < .001; **P < .01; *P < .05.