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. 2012 Oct 1;5(5):344–355. doi: 10.1593/tlo.12190

Figure 6.

Figure 6

Mechanistic study of the effect of L + E + P on cell growth, cell adhesion, and migration of hormone-independent prostate cancer cells. (A) PC3 cells were transfected with pcDNA3 CCNE2 vector (2 µg/ml) and treated with L + E + P at 8 µg/ml 24 hours after transfection. Cell number was counted at a 48-hour time point. (B) Immunoblot analysis for E-cadherin with protein extracts from PC3 cells treated with L + E + P at 8 µg/ml for 24 hours. (C) PC3 cells were transfected with 40 nM E-cadherin siRNA. Twenty-four hours after transfection, cells were treated with L + E + P at 8 µg/ml for 12 hours and adhesion assay was performed. PC3 cells were transfected with 2 µg/ml (D) pcDNA3.1 HMMR vector or (E) pcDNA4.1 TWIST vector and treated with L + E + P at 8 µg/ml 24 hours after transfection. Migrated distance was determined at a 48-hour time point. Bars represent SEM. **P < .01; *P < .05.